Ven. Lopon Thubten Gyaltsen was born on 15th December 1980 in Markham, Tibet. He became a Buddhist monk at the Tsechen Shedup Thekchokling monastery at the age of eight and began studying Buddhist Philosophy at the age of 14. He escaped from Tibet in 2000, joined Sakya Centre Rajpur, Dehradun, and studied higher Buddhist philosophy for 3 years. He then got enrolled at Sakya Institute Purawala and studied Sutra and Vajrayana for over ten years from numerous teachers including H. E. the Sakya Gongma Trizin Rinpoche. He attained degrees in Kachupa and Lopon and served as a Buddhist philosophy teacher at Sakya Institute for four years. He was nominated by H. E. the Sakya Gongma Trichen Rinpoche as a member of the Sakya settlement administration for three years. He also edited a dictionary for the Sakya editorial office in 2014 and was a member of the organizing committee for the long-life prayer offering to Kyabchok Ngor Sharchen Luding Khenchen Rinpoche in 2019.

He was earlier elected to the 16th Tibetan Parliament-in-Exile and was a member of the Standing Committee. He has actively participated in many official Tibet outreach programs. He is currently a member of the 17th Tibetan Parliament-in-Exile from the Sakya School of Tibetan Buddhism.